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Electrophoretic Analysis of Protein and Selected Enzymes of Acacia Seeds from Saudi Arabia

Ali A. Al-HELAl, JKAU:Sci., vol.6 pp . 17-28(1414 A.H./1994 A.D.)

The water-soluble, diluted salt-soluble and ethanol-soluble proteins of seed extracts from eight Saudi Arabia acacia species were analyzed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Qualitative differences were evident in the electrophoretic patterns of all protein fractions.

Also, the anodic isoenzyme patterns of amylase, glutamate oxaloacetate aminotransferase (GOT), non-specific esterase (EST) and phosphorylase in seed extracts were separated by polyacrylamide gel electrophoresis (PAGE). The studied enzymes were found to be highly polymorphic. It is clear from the results that the isoenzyme patterns can be used together with protein banding patterns as markers to estimate both genetic variation at biochemical level and taxonomical relationship among the species.